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マトリックスメタロプロテイナーゼ(MMP)アッセイシステム

代表的な測定マーカー:MMP、TIMP 

製品 コード番号 原理 包装 アッセイレンジ 感度 操作時間 備考
MMP-1, Human RPN2610 ELISA 96 wells 6.25~100 ng/ml

1.7 ng/ml 6.5 hr.
  • Specific for total MMP-1 (proMMP-1, active MMP-1 & MMP-1 / TIMP-1 complex)
  • Suitable for use with cell culture supernatant and serum / plasma samples
  • Sample volume: 100 μl
MMP-1 Activity Assay 製造終了品 RPN2629 MMP Activity Assay 96 wells 3.13~50 ng/ml
(1.5 hr. incubation with detection reagent)

0.1~1.56 ng/ml
(4.0 hr. incubation with detection reagent)

2.2 ng/ml

0.1 ng/ml

1.5 days
  • Measures endogenous active MMP-1 or total active MMP-1
  • Range / sensitivity: Equivalent to ELISA
  • Ease-of-use: Equivalent to ELISA
MMP-2, Human RPN2617 ELISA 96 wells 1.5~24 ng/ml 0.37 ng/ml 4.5 hr.
  • Specific for proMMP-2
    (free and complexed to TIMP-2), but not the active form of MMP-2
  • Suitable for use with cell culture supernatant, serum / plasma and tissue samples
  • Sample volume: 100 μl
MMP-2 Activity Assay RPN2631 MMP Activity Assay 96 wells 0.19~3.0 ng/ml
(4.0 hr. incubation with detection reagent)

0.75~12 ng/ml
(1.5 hr. incubation with detection reagent)
0.19 ng/ml

0.5 ng/ml
1.5 days
  • Suitable for use with cell culture supernatant, serum and plasma samples
  • Measures either endogenous (naturally occurring)or total active MMP-2(activated by APMA)
  • Sensitivity equivalent to ELISA / Zymography
  • Ease-of-use: Equivalent to ELISA
  • Sample volume: 100 μl
MMP-3 Activity Assay 製造終了品 RPN2639 MMP Activity Assay 96 wells 0.5~32 ng/ml
(1.5~2.5 hr. incubation with detection reagent)

0.125~8.0 ng/ml
(3.0~4.0 hr. incubation with detection reagent)
0.5 ng/ml

0.1 ng/ml
1.5 days
  • Measures either endogenous active MMP-3 (naturally occurring) or total active MMP-3 (following activation with APMA)
  • Quantitative
MMP-7, Human 製造終了品 RPN2620 ELISA 96 wells 0.31~10 ng/ml 97 pg/ml 1.5 days
  • Specific for human proMMP-7
  • Suitable for use with cell culture supernatants
  • Sample volume: 100 μl
MMP-8, Human RPN2619 ELISA 96 wells 0.25~4.0 ng/ml 32 pg/ml 4.5 hr.
  • Specific for:
    pro- and active MMP-8: 100%
    active MMP-8/ TIMP-1 complex: 50%
    active MMP-8/ TIMP-2 complex: 30%
  • Suitable for use with cell culture supernatant, serum / plasma and tissue samples
  • Sample volume: 100 μl
MMP-8 Activity Assay 製造終了品 RPN2635 MMP Activity Assay 96 wells 0.75~24 ng/ml
(2.0 hr. incubation with detection reagent)

0.09~6.0 ng/ml
(3.0~4.0 hr. incubation with detection reagent)
0.1 ng/ml 1.5 days
  • Measures either endogenous active MMP-8 or total active MMP-8 (activated by APMA)
  • Quantitative
MMP-9, Human RPN2614 ELISA 96 wells 1.0~32 ng/ml
(cell culture supernatant)

4.0~128 ng/ml
(plasma sample)
0.6 ng/ml 4.5 hr.
(cell culture supernatant)

2.5 hr. (plasma sample)
  • Specific for proMMP-9 (free and complexed to TIMP-1, TIMP-2)
  • Suitable for use with cell culture supernatant and plasma samples
  • Sample volume: 100 μl
MMP-9 Activity Assay RPN2634 MMP Activity Assay 96 wells 0.5~16 ng/ml
(1.0 hr. incubation with detection reagent)

0.125~4.0 ng/ml
(1.5 hr. incubation with detection reagent)
0.125 pg/ml 1.5 days
  • Measures endogenous active MMP-9 or total active MMP-9
  • Range / sensitivity: Equivalent to ELISA
  • Ease-of-use: Equivalent to ELISA
MMP-13, Human RPN2621 ELISA 96 wells 0.094~3.0 ng/ml 0.032 ng/ml 4.0 hr.
  • Specific for human pro- and active MMP-13
  • Suitable for use with cell culture supernatant, serum and plasma samples
  • Sample volume: 100 μl
MMP-14 Activity Assay 製造終了品 RPN2637 MMP Activity Assay 96 wells

1.0~32 ng/ml
(2.5 hr. incubation with detection reagent)

0.125~4.0 ng/ml
(6.0 hr. incubation with detection reagent)

0.7 ng/ml 1.5 days
  • Measures endogenous active MMP-14 (naturally occurring) in complex biological samples
  • Quantitative
TIMP-1, Human 製造終了品 RPNJ409 ELISA 96 wells 51~2,000 ng/ml 1.2 ng/ml 2.0 hr.
  • Suitable for use with urine, serum, plasma and synovial fluid.
  • Sample volume: 10 μl
  • 日本語取扱説明書添付
TIMP-2, Human RPN2618 ELISA 96 wells 8.0~128 ng/ml 3.0 ng/ml 3.5 hr.
  • Specific for free cell TIMP-2 and TIMP-2 complexed with active forms of MMPs, but not TIMP-2 complexed with pro-MMP-2 or to free TIMP-1 or free TIMP-3
  • Suitable for use with cell culture supernatant and serum / plasma and tissue samples
  • Sample volume: 100 μl


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